|
KMID : 0900920030270030249
|
|
Korean journal of Animal Reproduction
2003 Volume.27 No. 3 p.249 ~ p.258
|
|
|
Effects of Growth Factors and Hexoses on In Vitro Development in Porcine Embryos
|
|
Lee Sang-Young
Cheong Hee-Tae
Yang Boo-Keun
Kim Choung-Ik
Park Choon-Keun
|
|
|
Abstract
|
|
|
|
The aim of this study was to investigate the effects of different hexoses in medium with IGF-I and/or EGF on in vitro development of porcine embryos. In first experiment, when the embryos were cultured in medium with concentrations of 5 ng/ml IGF-I and 10 ng/ml EGF, the morula and blastocyst rates were higher than in another culture conditions (P<0.05). In second experiment, the effect of IGF-I in medium with different hexoses during the embryo culture was examined. The higher cleavage rates were obtained in medium with glucose and IGF-I (P<0.05). However, the higher proportion of embryos developed to morula and blastocyst stages in medium with IGF-I were obtained than in medium without IGF-I in medium with glucose, mannose and galactose. In third experiment, the effect of EGF on in vitro development of porcine embryos in medium with different hexoses were examined. In the culture medium was supplemented with glucose, the higher proportions (P<0.05) of embryos developed to molura and blastocyst stages were obtained in medium with that than without EGF. However, the proportions of embryos developed to blastocyst stage were not significantly different between with and without of EGF in medium with different hexoses. In firth experiment, the effects of presence of IGF-I and EGF in medium with different hexoses on in vitro development of porcine embryos were examined. When culture medium was supplemented with IGF-I and EGF, the higher proportions of oocytes cleaved and developed to 8-cell stage were obtained in medium with glucose than mannose, galactose and fructose (P<0.05). These results show that glucose and growth factors, supported the development in vitro of porcine embryos, especially with greater embryo cleavage and development in medium with glucose added to media with IGF-I and/or EGF.
|
|
|
KEYWORD
|
|
|
EGF, Hexoses, In vitro development, IGF-I, Porcine embryo
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|
|